We offer a number of services in molecular and cell biology and can help with experimental design, data analysis and interpretation.

    miRNA

    miRNA target search and pathway analysis

    miRNA RT-qPCR

    Cloning and verification of miRNA targets in the Luciferase assay

    Analysis of miRNA influence on endogenous targets

    miRNA in situ hybridization

    RNA

    total RNA isolation

    RT-qPCR

    Cell culture

    Human primary keratinocytes, melanocytes and fibroblasts

    Human reconstituted epidermis

    Bronchial epithelial cells

    PBMCs

    Human monocytes and monocyte derived dendritic cells and macrophages

    Primary T cells

    Human cell-lines (HeLa, HEK293, BEAS-2B, HaCat, A549, THP-1 and PPC-1)

     

    Molecular and cell biology

    Transfection of cell-lines, primary cells and T cells

    Proliferation and apoptosis assays

    Cloning

    Immunofluorescence analysis

    Western blot analysis

    Flow cytometry and cell sorting

     

    Data analysis and consultation

    Statistical data analysis using R

    Transcriptome analysis

    Visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets with „Imaris“ platform

    Research publishing

     

    Additional information http://www.biomeditsiin.ut.ee/en/departments/rna-biology-research-group-0